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phospho pi3k  (Abmart Inc)

 
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    Structured Review

    Abmart Inc phospho pi3k
    Phospho Pi3k, supplied by Abmart Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/phospho+pi3k/anti+p+pi3k/pmc13057433-116-29-31
    Average 86 stars, based on 1 article reviews
    phospho pi3k - by Bioz Stars, 2026-09
    86/100 stars

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    Related Articles

    Blocking Assay:

    Article Title: Novel Roles of GDF15 in Alleviating Renal Fibrosis: Promoting Autophagy and Lysosome Biogenesis via Inhibition of the PI3K /Akt/ mTOR Pathway
    Article Snippet: .. After blocking non‐specific binding with 5% BSA for 1 h, we incubated the membranes overnight at 4°C with primary antibodies against the following: collagen IA (1:1000; BA0325; Boster), Beclin1 (1:1000; T55092 ; Abmart), P62 (1:1000; T55546 ; Abmart), LC3B (1:1000; T55992 ; Abmart), total PI3K (1:1000; T40064 ; Abmart), phospho‐PI3K (1:1000; T40065 ; Abmart), total mTOR (1:1000; T55306 ; Abmart), phospho‐mTOR (1:1000; T56571 ; Abmart), TFEB (1:1000; TA7015; Abmart), total Akt (1:1000; 60203–2‐Ig; Proteintech), phospho‐Akt (1:1000; 66444–1‐Ig; Proteintech), fibronectin (1:1000; ab2413; Abcam), GDF15 (1:500; BS3818‐R; Bioss), and anti‐GFRAL (1:1000; ab214929; Abcam). .. Next, the membranes were exposed to the respective HRP‐conjugated secondary antibody (1:10000; EarthOx Life Sciences).

    Binding Assay:

    Article Title: Novel Roles of GDF15 in Alleviating Renal Fibrosis: Promoting Autophagy and Lysosome Biogenesis via Inhibition of the PI3K /Akt/ mTOR Pathway
    Article Snippet: .. After blocking non‐specific binding with 5% BSA for 1 h, we incubated the membranes overnight at 4°C with primary antibodies against the following: collagen IA (1:1000; BA0325; Boster), Beclin1 (1:1000; T55092 ; Abmart), P62 (1:1000; T55546 ; Abmart), LC3B (1:1000; T55992 ; Abmart), total PI3K (1:1000; T40064 ; Abmart), phospho‐PI3K (1:1000; T40065 ; Abmart), total mTOR (1:1000; T55306 ; Abmart), phospho‐mTOR (1:1000; T56571 ; Abmart), TFEB (1:1000; TA7015; Abmart), total Akt (1:1000; 60203–2‐Ig; Proteintech), phospho‐Akt (1:1000; 66444–1‐Ig; Proteintech), fibronectin (1:1000; ab2413; Abcam), GDF15 (1:500; BS3818‐R; Bioss), and anti‐GFRAL (1:1000; ab214929; Abcam). .. Next, the membranes were exposed to the respective HRP‐conjugated secondary antibody (1:10000; EarthOx Life Sciences).

    Incubation:

    Article Title: Novel Roles of GDF15 in Alleviating Renal Fibrosis: Promoting Autophagy and Lysosome Biogenesis via Inhibition of the PI3K /Akt/ mTOR Pathway
    Article Snippet: .. After blocking non‐specific binding with 5% BSA for 1 h, we incubated the membranes overnight at 4°C with primary antibodies against the following: collagen IA (1:1000; BA0325; Boster), Beclin1 (1:1000; T55092 ; Abmart), P62 (1:1000; T55546 ; Abmart), LC3B (1:1000; T55992 ; Abmart), total PI3K (1:1000; T40064 ; Abmart), phospho‐PI3K (1:1000; T40065 ; Abmart), total mTOR (1:1000; T55306 ; Abmart), phospho‐mTOR (1:1000; T56571 ; Abmart), TFEB (1:1000; TA7015; Abmart), total Akt (1:1000; 60203–2‐Ig; Proteintech), phospho‐Akt (1:1000; 66444–1‐Ig; Proteintech), fibronectin (1:1000; ab2413; Abcam), GDF15 (1:500; BS3818‐R; Bioss), and anti‐GFRAL (1:1000; ab214929; Abcam). .. Next, the membranes were exposed to the respective HRP‐conjugated secondary antibody (1:10000; EarthOx Life Sciences).

    Western Blot:

    Article Title: ERMP1 Exerts Tumor‐Suppressive Functions in KIRC by Inhibiting PI3K/AKT Signaling and Remodeling the Immune Microenvironment: A Pan‐Cancer Analysis
    Article Snippet: .. Primary antibodies and dilutions: ERMP1 (rabbit; Proteintech, 27224‐1‐AP; WB 1:2000; and IHC 1:100); Phospho‐Akt (Ser473) (rabbit; CST, 4060 T; and WB 1:2000); Akt (rabbit; Proteintech, 10176‐2‐AP; and WB 1:2000); Phospho‐PI3K (rabbit; Abmart, T40065S; and WB 1:2000); PI3K (rabbit; Abmart, T40064S; and WB 1:2000); N‐Cadherin (rabbit; Affinity, AF4039; and WB 1:2000); E‐Cadherin (rabbit; Affinity, AF0131; and WB 1:2000); MMP2 (rabbit; Proteintech, 10373‐2‐AP; and WB 1:2000); MMP9 (rabbit; Proteintech, 10375‐2‐AP; and WB 1:2000); Cyclin D1 (mouse; Proteintech, 60186‐1‐Ig; and WB 1:2000); CDK4 (rabbit; Proteintech, 11026‐1‐AP; and WB 1:2000); GAPDH (rabbit; Affinity, AF7021; and WB 1:2000). ..

    Article Title: Botulinum Neurotoxin Type A1 Alleviates Bleomycin-induced Skin Fibrosis by Inhibiting PI3K-Akt Pathway and Associated Cell Cycle and Proliferation.
    Article Snippet: This is a PDF of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability.. This version will undergo additional copyediting, typesetting and review before it is published in its final form.. As such, this version is no longer the Accepted Manuscript, but it is not yet the definitive Version of Record; we are providing this early version to give early visibility of the article.

    Immunohistochemistry:

    Article Title: ERMP1 Exerts Tumor‐Suppressive Functions in KIRC by Inhibiting PI3K/AKT Signaling and Remodeling the Immune Microenvironment: A Pan‐Cancer Analysis
    Article Snippet: .. Primary antibodies and dilutions: ERMP1 (rabbit; Proteintech, 27224‐1‐AP; WB 1:2000; and IHC 1:100); Phospho‐Akt (Ser473) (rabbit; CST, 4060 T; and WB 1:2000); Akt (rabbit; Proteintech, 10176‐2‐AP; and WB 1:2000); Phospho‐PI3K (rabbit; Abmart, T40065S; and WB 1:2000); PI3K (rabbit; Abmart, T40064S; and WB 1:2000); N‐Cadherin (rabbit; Affinity, AF4039; and WB 1:2000); E‐Cadherin (rabbit; Affinity, AF0131; and WB 1:2000); MMP2 (rabbit; Proteintech, 10373‐2‐AP; and WB 1:2000); MMP9 (rabbit; Proteintech, 10375‐2‐AP; and WB 1:2000); Cyclin D1 (mouse; Proteintech, 60186‐1‐Ig; and WB 1:2000); CDK4 (rabbit; Proteintech, 11026‐1‐AP; and WB 1:2000); GAPDH (rabbit; Affinity, AF7021; and WB 1:2000). ..



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    Image Search Results


    Tryptophanol treatment alleviated POF symptoms by inhibiting PI3K/AKT and MAPK pathways. A Experimental scheme. B Estrus status of mice ( n = 8). C Body weights of mice ( n = 8). D Ovary weights of mice ( n = 8). E Representative Ovary from the Control, the POF and the NCG groups mice with H&E and IHC staining. H&E scale bar = 200 μm; IHC scale bar = 100 μm. F Quantification of primordial, primary, secondary, antral follicles, corpora lutea and corpora atretica per ovary. n = 3 ovaries per each condition. Levels of FSH ( G ), LH ( H ), E 2 ( I ) in mouse serum ( n = 6). Levels of ROS ( J ), MDA ( K ), SOD ( L ), GSH-Px ( M ) in mouse ovaries ( n = 3–4). N Western blotting ( a ) and quantitative ( b ) analysis of proteins ( n = 3). O Litter sizes of mice ( n = 3). Data presented as the mean ± SD, statistical significance was determined using one-way or two-way ANOVA. * p < 0.05, ** p < 0.01. *** p < 0.001. Different lowercase letters indicate significant differences ( p < 0.05)

    Journal: Journal of Ovarian Research

    Article Title: Gut microbiota-derived Tryptophanol driven by N-Carbamylglutamate alleviates premature ovarian failure through inhibiting oxidative stress

    doi: 10.1186/s13048-026-02152-4

    Figure Lengend Snippet: Tryptophanol treatment alleviated POF symptoms by inhibiting PI3K/AKT and MAPK pathways. A Experimental scheme. B Estrus status of mice ( n = 8). C Body weights of mice ( n = 8). D Ovary weights of mice ( n = 8). E Representative Ovary from the Control, the POF and the NCG groups mice with H&E and IHC staining. H&E scale bar = 200 μm; IHC scale bar = 100 μm. F Quantification of primordial, primary, secondary, antral follicles, corpora lutea and corpora atretica per ovary. n = 3 ovaries per each condition. Levels of FSH ( G ), LH ( H ), E 2 ( I ) in mouse serum ( n = 6). Levels of ROS ( J ), MDA ( K ), SOD ( L ), GSH-Px ( M ) in mouse ovaries ( n = 3–4). N Western blotting ( a ) and quantitative ( b ) analysis of proteins ( n = 3). O Litter sizes of mice ( n = 3). Data presented as the mean ± SD, statistical significance was determined using one-way or two-way ANOVA. * p < 0.05, ** p < 0.01. *** p < 0.001. Different lowercase letters indicate significant differences ( p < 0.05)

    Article Snippet: The membrane was blocked with 5% bovine serum albumin (BSA; Sigma) for 1 h at room temperature, followed by overnight incubation at 4 °C with the primary antibodies: rabbit Cleaved caspase 3 (MCE, HY- P80623 , 1:800), rabbit BAX (Bioworld, BS61098, 1:800), rabbit BCL2 (Bioworld, BS1511, 1:800), rabbit ERK1/2 (Bioworld, BS90472, 1:1,000), rabbit p-ERK1/2 (Bioworld, BS4621P, 1:1,000), rabbit p38 (Bioworld, BS4635, 1:1,000), rabbit p-p38 (Bioworld, BS4766, 1:1,000), rabbit PI3K (MCE, HY- P80867 , 1:1,000); rabbit p-PI3K (MCE, HY- P80846 , 1:1,000), rabbit AKT (Bioworld, MB3288 1:1,000); rabbit p-AKT (Bioworld, BS4007 1:1,000); rabbit IL-6 (Abclonal, A0286, 1:800), rabbit IL-1β (MCE, HY- P80720 , 1:1,000), rabbit Occludin (Abclonal, A2601, 1:1,000); rabbit ZO-1 (Bioworld, BS71522, 1:1,000); rabbit GPX4 (MCE, HY- P80450 , 1:1,000); mouse GAPDH (Bioworld, MB001, 1:1,000); rabbit SOD2 (Bioworld, BS6734, 1:1,000).

    Techniques: Control, Immunohistochemistry, Western Blot

    Tryptophanol treatment alleviated POF by inhibiting PI3K/AKT and MAPK pathways in vitro . A Experimental scheme. B Different concentrations of Cy on GCs viability was assessed by CCK8 assay. C and E Different concentrations of Trp on GCs viability was assessed by CCK8 assay. D The effect of Trp on GCs cell viability following Cy treatment. The control and treatment groups were stained with DCFH-DA, the cells were washed and examined by fluorescence microscopy. Scale bar = 50 μm. F The fluorescence intensity of ROS in mouse GCs. G The level of MDA in mouse GCs. H Flow cytometry apoptosis analysis graph and cell death rate in different groups. The level of SOD ( I ), GSH-Px ( J ), and E 2 ( K ) in mouse GCs. ( L ) Western blotting ( a ) and quantitative ( b ) analysis of proteins ( n = 3). All values are presented as the mean ± SD ( n = 3). Different lowercase letters indicate significant differences compared to the control group ( p < 0.05)

    Journal: Journal of Ovarian Research

    Article Title: Gut microbiota-derived Tryptophanol driven by N-Carbamylglutamate alleviates premature ovarian failure through inhibiting oxidative stress

    doi: 10.1186/s13048-026-02152-4

    Figure Lengend Snippet: Tryptophanol treatment alleviated POF by inhibiting PI3K/AKT and MAPK pathways in vitro . A Experimental scheme. B Different concentrations of Cy on GCs viability was assessed by CCK8 assay. C and E Different concentrations of Trp on GCs viability was assessed by CCK8 assay. D The effect of Trp on GCs cell viability following Cy treatment. The control and treatment groups were stained with DCFH-DA, the cells were washed and examined by fluorescence microscopy. Scale bar = 50 μm. F The fluorescence intensity of ROS in mouse GCs. G The level of MDA in mouse GCs. H Flow cytometry apoptosis analysis graph and cell death rate in different groups. The level of SOD ( I ), GSH-Px ( J ), and E 2 ( K ) in mouse GCs. ( L ) Western blotting ( a ) and quantitative ( b ) analysis of proteins ( n = 3). All values are presented as the mean ± SD ( n = 3). Different lowercase letters indicate significant differences compared to the control group ( p < 0.05)

    Article Snippet: The membrane was blocked with 5% bovine serum albumin (BSA; Sigma) for 1 h at room temperature, followed by overnight incubation at 4 °C with the primary antibodies: rabbit Cleaved caspase 3 (MCE, HY- P80623 , 1:800), rabbit BAX (Bioworld, BS61098, 1:800), rabbit BCL2 (Bioworld, BS1511, 1:800), rabbit ERK1/2 (Bioworld, BS90472, 1:1,000), rabbit p-ERK1/2 (Bioworld, BS4621P, 1:1,000), rabbit p38 (Bioworld, BS4635, 1:1,000), rabbit p-p38 (Bioworld, BS4766, 1:1,000), rabbit PI3K (MCE, HY- P80867 , 1:1,000); rabbit p-PI3K (MCE, HY- P80846 , 1:1,000), rabbit AKT (Bioworld, MB3288 1:1,000); rabbit p-AKT (Bioworld, BS4007 1:1,000); rabbit IL-6 (Abclonal, A0286, 1:800), rabbit IL-1β (MCE, HY- P80720 , 1:1,000), rabbit Occludin (Abclonal, A2601, 1:1,000); rabbit ZO-1 (Bioworld, BS71522, 1:1,000); rabbit GPX4 (MCE, HY- P80450 , 1:1,000); mouse GAPDH (Bioworld, MB001, 1:1,000); rabbit SOD2 (Bioworld, BS6734, 1:1,000).

    Techniques: In Vitro, CCK-8 Assay, Control, Staining, Fluorescence, Microscopy, Flow Cytometry, Western Blot